Three-phase partitioning (TPP) is an efficient and cost-effective method for protein purification that exploits the simultaneous addition of salts and organic solvents to an aqueous protein extract.

Affinity chromatography has emerged as an indispensable tool in modern protein purification, leveraging highly selective interactions between target proteins and immobilised ligands. This technique, ...

Proteins are bio-macromolecules with a range of functionalities as a result of complex higher-order structures. As the end product of the “central dogma” in biology, proteins are considered the key ...

Depending on the process and who provides the statistics, downstream processing accounts for between 50–80% of the cost of producing biotech drugs. Whatever the exact number, protein purification ...

Protein samples are essential to advance our understanding of disease pathways and develop new therapeutics. High-quality filtration is needed to protect purity, yield and functionality of target ...

Conventionally, chromatography is used for protein purification, which is extremely expensive. Alternatively, the protein crystallization method could be used to determine protein structure, but this ...

Self-removing tags can be used for simple and effective purification of recombinant proteins and peptides. The key component of these tags is an intein, which is a protein element with the ability to ...

There are a wide variety of protein-based drugs that are used to treat various serious conditions. Insulin is perhaps the most well-known example, which is used for life-saving treatments for diabetes ...

So-called ‘alternative proteins’ – from upcycled barley and canola protein to animal-free whey made in fermentation tanks using genetically engineered microbes – were a hot topic at IFT First in ...